ssr_primer
Primer Details for TRINITY_DN83645_c7_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATGGCTGGTTTTGGCAG | ATGTCCGGGCCTGTTTTCAT | Start: 1966 |
| Tm | 60.037 | 59.96 | End: 2135 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | CCTAGCCACATGTCCACTGG | TGAAAACTTGCGGAACAGCG | Start: 252 |
| Tm | 60.108 | 59.971 | End: 502 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
|---|
| Sequence | CCTCCTAGCCACATGTCCAC | TGAAAACTTGCGGAACAGCG | Start: 249 |
| Tm | 59.821 | 59.971 | End: 502 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | CACCTCCTAGCCACATGTCC | TGAAAACTTGCGGAACAGCG | Start: 247 |
| Tm | 59.821 | 59.971 | End: 502 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | GGAATGGCTGGTTTTGGCAG | CGAGACAGCAAGACCTCCTC | Start: 1966 |
| Tm | 60.037 | 59.828 | End: 2234 |
| Size | 20 | 20 | 269 |
(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATGGCTGGTTTTGGCAG | ATGTCCGGGCCTGTTTTCAT | Start: 1966 |
| Tm | 60.037 | 59.96 | End: 2135 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | CCTAGCCACATGTCCACTGG | TGAAAACTTGCGGAACAGCG | Start: 252 |
| Tm | 60.108 | 59.971 | End: 502 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
|---|
| Sequence | CCTCCTAGCCACATGTCCAC | TGAAAACTTGCGGAACAGCG | Start: 249 |
| Tm | 59.821 | 59.971 | End: 502 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | CACCTCCTAGCCACATGTCC | TGAAAACTTGCGGAACAGCG | Start: 247 |
| Tm | 59.821 | 59.971 | End: 502 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | GGAATGGCTGGTTTTGGCAG | CGAGACAGCAAGACCTCCTC | Start: 1966 |
| Tm | 60.037 | 59.828 | End: 2234 |
| Size | 20 | 20 | 269 |
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