ssr_primer
Primer Details for TRINITY_DN83751_c3_g1_i1 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGATGCGTTTTCCCCCTT | ATAGAGGTGGCAAGACGCAC | Start: 749 |
| Tm | 59.888 | 60.108 | End: 998 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | AGGTCCAAGAATGAGCCGTG | Start: 819 |
| Tm | 59.602 | 60.036 | End: 1041 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | GGTCCAAGAATGAGCCGTGA | Start: 819 |
| Tm | 59.602 | 60.036 | End: 1040 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | ATAGAGGTGGCAAGACGCAC | Start: 819 |
| Tm | 59.602 | 60.108 | End: 998 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | GAGGTCCAAGAATGAGCCGT | Start: 819 |
| Tm | 59.602 | 59.749 | End: 1042 |
| Size | 20 | 20 | 224 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGATGCGTTTTCCCCCTT | ATAGAGGTGGCAAGACGCAC | Start: 749 |
| Tm | 59.888 | 60.108 | End: 998 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | AGGTCCAAGAATGAGCCGTG | Start: 819 |
| Tm | 59.602 | 60.036 | End: 1041 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | GGTCCAAGAATGAGCCGTGA | Start: 819 |
| Tm | 59.602 | 60.036 | End: 1040 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | ATAGAGGTGGCAAGACGCAC | Start: 819 |
| Tm | 59.602 | 60.108 | End: 998 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGACAGACAAGCCATGCTCA | GAGGTCCAAGAATGAGCCGT | Start: 819 |
| Tm | 59.602 | 59.749 | End: 1042 |
| Size | 20 | 20 | 224 |
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