ssr_primer
Primer Details for TRINITY_DN84008_c4_g1_i3 |
|---|
| (T)12attccc(T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGAGATGCACTGCCTGGG | GGCTGGAAGGTGAAGGTTCA | Start: 508 |
| Tm | 59.894 | 59.89 | End: 669 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | CTAGAGATGCACTGCCTGGG | TGGCTGGAAGGTGAAGGTTC | Start: 508 |
| Tm | 59.894 | 59.89 | End: 670 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GAACCTTCACCTTCCAGCCA | TGCCAAAATATCGCAACCGC | Start: 651 |
| Tm | 59.89 | 60.179 | End: 926 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGAACCTTCACCTTCCAGCC | TGCCAAAATATCGCAACCGC | Start: 650 |
| Tm | 59.89 | 60.179 | End: 926 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | GTTGGCAGACCCAGGGATTT | GGCTGGAAGGTGAAGGTTCA | Start: 527 |
| Tm | 60.252 | 59.89 | End: 669 |
| Size | 20 | 20 | 143 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGAGATGCACTGCCTGGG | GGCTGGAAGGTGAAGGTTCA | Start: 508 |
| Tm | 59.894 | 59.89 | End: 669 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | CTAGAGATGCACTGCCTGGG | TGGCTGGAAGGTGAAGGTTC | Start: 508 |
| Tm | 59.894 | 59.89 | End: 670 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GAACCTTCACCTTCCAGCCA | TGCCAAAATATCGCAACCGC | Start: 651 |
| Tm | 59.89 | 60.179 | End: 926 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGAACCTTCACCTTCCAGCC | TGCCAAAATATCGCAACCGC | Start: 650 |
| Tm | 59.89 | 60.179 | End: 926 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | GTTGGCAGACCCAGGGATTT | GGCTGGAAGGTGAAGGTTCA | Start: 527 |
| Tm | 60.252 | 59.89 | End: 669 |
| Size | 20 | 20 | 143 |
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