ssr_primer
Primer Details for TRINITY_DN84214_c3_g1_i9 |
|---|
| (TCT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGATCTTGCGGCAATGAA | ACTTGCATCAACCAACGCAC | Start: 974 |
| Tm | 60.034 | 59.969 | End: 1138 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | AAGGGATCTTGCGGCAATGA | ACTTGCATCAACCAACGCAC | Start: 973 |
| Tm | 60.034 | 59.969 | End: 1138 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | TCTACTTCCGGCTCGTCTGA | ACTTGCATCAACCAACGCAC | Start: 859 |
| Tm | 60.036 | 59.969 | End: 1138 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | GTGCGTTGGTTGATGCAAGT | AGCGCATGGACTGAAAAGGA | Start: 1119 |
| Tm | 59.969 | 59.963 | End: 1265 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | TCTACTTCCGGCTCGTCTGA | TCATTGCCGCAAGATCCCTT | Start: 859 |
| Tm | 60.036 | 60.034 | End: 992 |
| Size | 20 | 20 | 134 |
(T)38: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGATCTTGCGGCAATGAA | ACTTGCATCAACCAACGCAC | Start: 974 |
| Tm | 60.034 | 59.969 | End: 1138 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | AAGGGATCTTGCGGCAATGA | ACTTGCATCAACCAACGCAC | Start: 973 |
| Tm | 60.034 | 59.969 | End: 1138 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | TCTACTTCCGGCTCGTCTGA | ACTTGCATCAACCAACGCAC | Start: 859 |
| Tm | 60.036 | 59.969 | End: 1138 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | GTGCGTTGGTTGATGCAAGT | AGCGCATGGACTGAAAAGGA | Start: 1119 |
| Tm | 59.969 | 59.963 | End: 1265 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | TCTACTTCCGGCTCGTCTGA | TCATTGCCGCAAGATCCCTT | Start: 859 |
| Tm | 60.036 | 60.034 | End: 992 |
| Size | 20 | 20 | 134 |
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