ssr_primer
Primer Details for TRINITY_DN84229_c2_g1_i3 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGAGCGAATCTCCGAGAG | CCGACTGGACCTGTCAACTC | Start: 74 |
| Tm | 59.97 | 60.038 | End: 266 |
| Size | 20 | 20 | 193 |
| Primer 2 details |
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| Sequence | GGGGTCAAGTTATGCCACGA | CCGACTGGACCTGTCAACTC | Start: 52 |
| Tm | 60.036 | 60.038 | End: 266 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | GGTGAGCGAATCTCCGAGAG | TGCAAGATGGTAGGGGGAGA | Start: 74 |
| Tm | 59.97 | 59.956 | End: 296 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
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| Sequence | GGTGGGGTCAAGTTATGCCA | CCGACTGGACCTGTCAACTC | Start: 49 |
| Tm | 59.961 | 60.038 | End: 266 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
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| Sequence | GAGCGAATCTCCGAGAGAGC | CCGACTGGACCTGTCAACTC | Start: 77 |
| Tm | 60.04 | 60.038 | End: 266 |
| Size | 20 | 20 | 190 |
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