ssr_primer
Primer Details for TRINITY_DN84385_c3_g2_i2 |
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| (T)11gcttttggtcgagagagtggtcagaa(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCGTCCGGTCAAATGTAA | TGAGCCCTTTTCATAGCGCA | Start: 1847 |
| Tm | 59.965 | 60.036 | End: 2083 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
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| Sequence | CCCCTTAAACACTCCCCGTC | TGCTGGAGCCTTTGTCTGAG | Start: 801 |
| Tm | 60.036 | 59.964 | End: 1048 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
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| Sequence | TTCTGCGGGTCCAGAAAGTC | GCACTTGGAAGTTGCTTGCA | Start: 3158 |
| Tm | 59.965 | 59.897 | End: 3257 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | ATGACCAAACTGTGTTGCGC | TCAGCTCAGATCACAGCAGC | Start: 3694 |
| Tm | 59.969 | 60.108 | End: 3833 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
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| Sequence | CATACGGTGTGCAGAGGTGT | GCACTTGGAAGTTGCTTGCA | Start: 3121 |
| Tm | 60.037 | 59.897 | End: 3257 |
| Size | 20 | 20 | 137 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCGTCCGGTCAAATGTAA | TGAGCCCTTTTCATAGCGCA | Start: 1847 |
| Tm | 59.965 | 60.036 | End: 2083 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
|---|
| Sequence | CCCCTTAAACACTCCCCGTC | TGCTGGAGCCTTTGTCTGAG | Start: 801 |
| Tm | 60.036 | 59.964 | End: 1048 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TTCTGCGGGTCCAGAAAGTC | GCACTTGGAAGTTGCTTGCA | Start: 3158 |
| Tm | 59.965 | 59.897 | End: 3257 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | ATGACCAAACTGTGTTGCGC | TCAGCTCAGATCACAGCAGC | Start: 3694 |
| Tm | 59.969 | 60.108 | End: 3833 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | CATACGGTGTGCAGAGGTGT | GCACTTGGAAGTTGCTTGCA | Start: 3121 |
| Tm | 60.037 | 59.897 | End: 3257 |
| Size | 20 | 20 | 137 |
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