ssr_primer
Primer Details for TRINITY_DN85027_c2_g1_i2 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGCACGGCAGAGTAGTAC | CAAGGGAGCTCACACCGTAA | Start: 177 |
| Tm | 59.83 | 59.68 | End: 425 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | TTCGCACGGCAGAGTAGTAC | TTACAAGGGAGCTCACACCG | Start: 177 |
| Tm | 59.83 | 59.68 | End: 428 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | CTTCGCACGGCAGAGTAGTA | CAAGGGAGCTCACACCGTAA | Start: 176 |
| Tm | 59.549 | 59.68 | End: 425 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
|---|
| Sequence | CTTCGCACGGCAGAGTAGTA | TTACAAGGGAGCTCACACCG | Start: 176 |
| Tm | 59.549 | 59.68 | End: 428 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GTTTTTCCTTCGCACGGCAG | CAAGGGAGCTCACACCGTAA | Start: 169 |
| Tm | 60.66 | 59.68 | End: 425 |
| Size | 20 | 20 | 257 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGCACGGCAGAGTAGTAC | CAAGGGAGCTCACACCGTAA | Start: 177 |
| Tm | 59.83 | 59.68 | End: 425 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | TTCGCACGGCAGAGTAGTAC | TTACAAGGGAGCTCACACCG | Start: 177 |
| Tm | 59.83 | 59.68 | End: 428 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | CTTCGCACGGCAGAGTAGTA | CAAGGGAGCTCACACCGTAA | Start: 176 |
| Tm | 59.549 | 59.68 | End: 425 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
|---|
| Sequence | CTTCGCACGGCAGAGTAGTA | TTACAAGGGAGCTCACACCG | Start: 176 |
| Tm | 59.549 | 59.68 | End: 428 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GTTTTTCCTTCGCACGGCAG | CAAGGGAGCTCACACCGTAA | Start: 169 |
| Tm | 60.66 | 59.68 | End: 425 |
| Size | 20 | 20 | 257 |
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