ssr_primer
Primer Details for TRINITY_DN85270_c5_g1_i1 |
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| (TCA)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTGTTCCACGCATATTGG | TCTGAAACCGCACCTGACTC | Start: 1727 |
| Tm | 59.972 | 59.966 | End: 1868 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
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| Sequence | CTTGGTGGCAGGATCTGTGT | TCTGAAACCGCACCTGACTC | Start: 1749 |
| Tm | 59.963 | 59.966 | End: 1868 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
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| Sequence | ACTTGGTGGCAGGATCTGTG | TCTGAAACCGCACCTGACTC | Start: 1748 |
| Tm | 59.963 | 59.966 | End: 1868 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | TTCCTTCGCCTCTTGCTCAG | TTAAGGAAGCTGAAGCCGCA | Start: 519 |
| Tm | 60.037 | 59.964 | End: 655 |
| Size | 20 | 20 | 137 |
| Primer 5 details |
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| Sequence | TGCGGCTTCAGCTTCCTTAA | GGGCGAAACACGAGCAAATT | Start: 636 |
| Tm | 59.964 | 60.04 | End: 835 |
| Size | 20 | 20 | 200 |
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