ssr_primer
Primer Details for TRINITY_DN85317_c4_g3_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTGTGGAGGAGGGAAGCG | ACTTATCTCCTCGCTGCGTG | Start: 79 |
| Tm | 60.035 | 59.899 | End: 253 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
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| Sequence | GGAGGAGGGAAGCGTTTCTT | ACTTATCTCCTCGCTGCGTG | Start: 85 |
| Tm | 59.675 | 59.899 | End: 253 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
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| Sequence | AATTGTGGAGGAGGGAAGCG | GTGCGTTACGGAGATCCTCA | Start: 79 |
| Tm | 60.035 | 59.545 | End: 236 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
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| Sequence | GTGGAGGAGGGAAGCGTTTC | ACTTATCTCCTCGCTGCGTG | Start: 83 |
| Tm | 60.674 | 59.899 | End: 253 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | GGAGGAGGGAAGCGTTTCTT | GTGCGTTACGGAGATCCTCA | Start: 85 |
| Tm | 59.675 | 59.545 | End: 236 |
| Size | 20 | 20 | 152 |
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