ssr_primer
Primer Details for TRINITY_DN85366_c2_g1_i8 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTCGAAAGACCGTCTCCT | TGGTCTATCCTGGATGCCGA | Start: 127 |
| Tm | 59.756 | 60.106 | End: 358 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
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| Sequence | TCGGCATCCAGGATAGACCA | TCTTAGGATGCCGCCGAAAA | Start: 339 |
| Tm | 60.106 | 59.749 | End: 574 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
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| Sequence | TTTTCGGCGGCATCCTAAGA | ACACAAGGTTCCGATGCACT | Start: 555 |
| Tm | 59.749 | 59.891 | End: 664 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | CGGAGACTGGAGAGTAGGGT | TGGTCTATCCTGGATGCCGA | Start: 165 |
| Tm | 59.744 | 60.106 | End: 358 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
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| Sequence | GCTTCGAAAGACCGTCTCCT | ACCCTACTCTCCAGTCTCCG | Start: 127 |
| Tm | 59.756 | 59.744 | End: 402 |
| Size | 20 | 20 | 276 |
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