ssr_primer
Primer Details for TRINITY_DN85474_c0_g3_i3 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGAGGAGGTTTTGGCAATG | AAACACCCAACAGCTTGGTG | Start: 619 |
| Tm | 60.109 | 59.177 | End: 885 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
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| Sequence | AGCGAGGAGGTTTTGGCAAT | AAACACCCAACAGCTTGGTG | Start: 618 |
| Tm | 60.251 | 59.177 | End: 885 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
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| Sequence | GCGAGGAGGTTTTGGCAATG | TGAAACACCCAACAGCTTGG | Start: 619 |
| Tm | 60.109 | 58.89 | End: 887 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | GCGAGGAGGTTTTGGCAATG | GAAACACCCAACAGCTTGGT | Start: 619 |
| Tm | 60.109 | 58.889 | End: 886 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
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| Sequence | GAGAGAGAAAGGATCGCTGCA | CCGAGAGCAATCCCGAACTT | Start: 21 |
| Tm | 59.864 | 60.108 | End: 176 |
| Size | 21 | 20 | 156 |
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