ssr_primer
Primer Details for TRINITY_DN85481_c1_g2_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCTCTGATGAACTCGGGT | TGCTCCCCTTCTTTTGCTGT | Start: 151 |
| Tm | 60.252 | 59.814 | End: 393 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
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| Sequence | CGCAAGGAAATCAAGCGGAA | TGCTCCCCTTCTTTTGCTGT | Start: 214 |
| Tm | 59.479 | 59.814 | End: 393 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
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| Sequence | CGCAAGGAAATCAAGCGGAA | TTCCCTTTCCTCCCTCCTCA | Start: 214 |
| Tm | 59.479 | 59.504 | End: 453 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
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| Sequence | TGCCTGGCCTCACATACTAC | ACCCGAGTTCATCAGAGGGT | Start: 0 |
| Tm | 59.169 | 60.252 | End: 170 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
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| Sequence | CAAGCGGAAGAGGAAGGAGA | TGCTCCCCTTCTTTTGCTGT | Start: 225 |
| Tm | 59.102 | 59.814 | End: 393 |
| Size | 20 | 20 | 169 |
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