ssr_primer
Primer Details for TRINITY_DN85535_c3_g4_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTCTAGGTGCGTTTGACT | CCTTGCGCCGAATGTATGTG | Start: 245 |
| Tm | 59.661 | 59.972 | End: 390 |
| Size | 21 | 20 | 146 |
| Primer 2 details |
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| Sequence | TCGTTCTAGGTGCGTTTGACT | GCCTCTCTGTAACTCCTCGC | Start: 245 |
| Tm | 59.661 | 59.898 | End: 409 |
| Size | 21 | 20 | 165 |
| Primer 3 details |
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| Sequence | TCGTTCTAGGTGCGTTTGACT | TGCGCCGAATGTATGTGAGT | Start: 245 |
| Tm | 59.661 | 60.108 | End: 387 |
| Size | 21 | 20 | 143 |
| Primer 4 details |
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| Sequence | TCGTTCTAGGTGCGTTTGACT | TCTGTAACTCCTCGCCTTGC | Start: 245 |
| Tm | 59.661 | 59.753 | End: 404 |
| Size | 21 | 20 | 160 |
| Primer 5 details |
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| Sequence | GTCGTAGGGACCAAACCTCC | AGTCAAACGCACCTAGAACGA | Start: 66 |
| Tm | 59.751 | 59.661 | End: 265 |
| Size | 20 | 21 | 200 |
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