ssr_primer
Primer Details for TRINITY_DN85663_c1_g2_i1 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGGTTTCCCTTAGGCC | CTATCGGACGGTTAAGGGCC | Start: 173 |
| Tm | 59.961 | 59.967 | End: 393 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | CGGTTTCCCTTAGGCCGTTA | CTATCGGACGGTTAAGGGCC | Start: 177 |
| Tm | 59.75 | 59.967 | End: 393 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | CCCCATCGATGCACGTTAGT | ACCGTAACCGATGCATTCCA | Start: 12 |
| Tm | 60.179 | 59.749 | End: 127 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | TTTCCGGTTTCCCTTAGGCC | TCGGACGGTTAAGGGCCTAT | Start: 173 |
| Tm | 59.961 | 60.398 | End: 390 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | TTTCCGGTTTCCCTTAGGCC | ATCGGACGGTTAAGGGCCTA | Start: 173 |
| Tm | 59.961 | 60.398 | End: 391 |
| Size | 20 | 20 | 219 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGGTTTCCCTTAGGCC | CTATCGGACGGTTAAGGGCC | Start: 173 |
| Tm | 59.961 | 59.967 | End: 393 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | CGGTTTCCCTTAGGCCGTTA | CTATCGGACGGTTAAGGGCC | Start: 177 |
| Tm | 59.75 | 59.967 | End: 393 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | CCCCATCGATGCACGTTAGT | ACCGTAACCGATGCATTCCA | Start: 12 |
| Tm | 60.179 | 59.749 | End: 127 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | TTTCCGGTTTCCCTTAGGCC | TCGGACGGTTAAGGGCCTAT | Start: 173 |
| Tm | 59.961 | 60.398 | End: 390 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | TTTCCGGTTTCCCTTAGGCC | ATCGGACGGTTAAGGGCCTA | Start: 173 |
| Tm | 59.961 | 60.398 | End: 391 |
| Size | 20 | 20 | 219 |
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