ssr_primer
Primer Details for TRINITY_DN85756_c2_g2_i3 |
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| (T)26: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCTGCGGCATTTAGAGGA | TATGGTCTCAGCGCACATCG | Start: 22 |
| Tm | 60.179 | 60.249 | End: 185 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
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| Sequence | GAGCTGCGGCATTTAGAGGA | GGTCTCAGCGCACATCGTTA | Start: 22 |
| Tm | 60.179 | 60.458 | End: 182 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
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| Sequence | TGAGCTGCGGCATTTAGAGG | TATGGTCTCAGCGCACATCG | Start: 21 |
| Tm | 60.463 | 60.249 | End: 185 |
| Size | 20 | 20 | 165 |
| Primer 4 details |
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| Sequence | AGCTGCGGCATTTAGAGGAT | TATGGTCTCAGCGCACATCG | Start: 23 |
| Tm | 59.528 | 60.249 | End: 185 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | GAGCTGCGGCATTTAGAGGA | GCTTTCGCTTCTGTTGACGT | Start: 22 |
| Tm | 60.179 | 59.418 | End: 218 |
| Size | 20 | 20 | 197 |
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