ssr_primer
Primer Details for TRINITY_DN86121_c0_g2_i3 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTACACTCGCAAACACCA | GACTGGCAGGCACCTAACAT | Start: 1244 |
| Tm | 59.894 | 60.035 | End: 1460 |
| Size | 20 | 20 | 217 |
| Primer 2 details |
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| Sequence | TTTGACTTCAGGGGTGCGTT | GGGAAGGAGAAAGACAGGCC | Start: 5416 |
| Tm | 60.107 | 60.035 | End: 5549 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | TTTGACTTCAGGGGTGCGTT | GCAAAAAGCGCCAGATTGGA | Start: 5416 |
| Tm | 60.107 | 60.038 | End: 5525 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | GCACCTTGGTGGCTAGGAAT | GGAAAGGCGAATTGAACGGG | Start: 5063 |
| Tm | 60.034 | 59.83 | End: 5300 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
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| Sequence | CCCGTTCAATTCGCCTTTCC | GGGAAGGAGAAAGACAGGCC | Start: 5281 |
| Tm | 59.83 | 60.035 | End: 5549 |
| Size | 20 | 20 | 269 |
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