ssr_primer
Primer Details for TRINITY_DN86407_c1_g3_i1 |
|---|
| (TC)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCTCTCTCTTCCGCCCTC | GACCGCCCGCAATCCTATAG | Start: 131 |
| Tm | 60.107 | 60.39 | End: 389 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | TCCGCCCTCCCGTATATTCT | GACCGCCCGCAATCCTATAG | Start: 142 |
| Tm | 59.886 | 60.39 | End: 389 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TCTTCCGCCCTCCCGTATAT | GACCGCCCGCAATCCTATAG | Start: 139 |
| Tm | 59.886 | 60.39 | End: 389 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | ACCTACGCTGAAACATCGCT | TCCAGCGCACGATTTCTTCA | Start: 436 |
| Tm | 59.753 | 60.32 | End: 666 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
|---|
| Sequence | ACCTACGCTGAAACATCGCT | TTTGCTTCTCCCCGACTCTG | Start: 436 |
| Tm | 59.753 | 59.678 | End: 614 |
| Size | 20 | 20 | 179 |
(A)27: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCTCTCTCTTCCGCCCTC | GACCGCCCGCAATCCTATAG | Start: 131 |
| Tm | 60.107 | 60.39 | End: 389 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | TCCGCCCTCCCGTATATTCT | GACCGCCCGCAATCCTATAG | Start: 142 |
| Tm | 59.886 | 60.39 | End: 389 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TCTTCCGCCCTCCCGTATAT | GACCGCCCGCAATCCTATAG | Start: 139 |
| Tm | 59.886 | 60.39 | End: 389 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | ACCTACGCTGAAACATCGCT | TCCAGCGCACGATTTCTTCA | Start: 436 |
| Tm | 59.753 | 60.32 | End: 666 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
|---|
| Sequence | ACCTACGCTGAAACATCGCT | TTTGCTTCTCCCCGACTCTG | Start: 436 |
| Tm | 59.753 | 59.678 | End: 614 |
| Size | 20 | 20 | 179 |
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