ssr_primer
Primer Details for TRINITY_DN86415_c3_g5_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGCCCATTCAGCTTTTC | GACGGCCGGAGAAGAAGAAA | Start: 294 |
| Tm | 60.109 | 60.038 | End: 518 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TTTCTTCTTCTCCGGCCGTC | AGACAAATCGTCCTCGGCTC | Start: 499 |
| Tm | 60.038 | 59.826 | End: 609 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | CGGTGCCCATTCAGCTTTTC | CCTGTACGAATGCAGCTCCA | Start: 294 |
| Tm | 60.109 | 60.108 | End: 472 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | TGGAGCTGCATTCGTACAGG | AGACAAATCGTCCTCGGCTC | Start: 453 |
| Tm | 60.108 | 59.826 | End: 609 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | GGTGCCCATTCAGCTTTTCC | GACGGCCGGAGAAGAAGAAA | Start: 295 |
| Tm | 59.753 | 60.038 | End: 518 |
| Size | 20 | 20 | 224 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGCCCATTCAGCTTTTC | GACGGCCGGAGAAGAAGAAA | Start: 294 |
| Tm | 60.109 | 60.038 | End: 518 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TTTCTTCTTCTCCGGCCGTC | AGACAAATCGTCCTCGGCTC | Start: 499 |
| Tm | 60.038 | 59.826 | End: 609 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | CGGTGCCCATTCAGCTTTTC | CCTGTACGAATGCAGCTCCA | Start: 294 |
| Tm | 60.109 | 60.108 | End: 472 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | TGGAGCTGCATTCGTACAGG | AGACAAATCGTCCTCGGCTC | Start: 453 |
| Tm | 60.108 | 59.826 | End: 609 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | GGTGCCCATTCAGCTTTTCC | GACGGCCGGAGAAGAAGAAA | Start: 295 |
| Tm | 59.753 | 60.038 | End: 518 |
| Size | 20 | 20 | 224 |
(C)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGCCCATTCAGCTTTTC | GACGGCCGGAGAAGAAGAAA | Start: 294 |
| Tm | 60.109 | 60.038 | End: 518 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TTTCTTCTTCTCCGGCCGTC | AGACAAATCGTCCTCGGCTC | Start: 499 |
| Tm | 60.038 | 59.826 | End: 609 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | CGGTGCCCATTCAGCTTTTC | CCTGTACGAATGCAGCTCCA | Start: 294 |
| Tm | 60.109 | 60.108 | End: 472 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | TGGAGCTGCATTCGTACAGG | AGACAAATCGTCCTCGGCTC | Start: 453 |
| Tm | 60.108 | 59.826 | End: 609 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | GGTGCCCATTCAGCTTTTCC | GACGGCCGGAGAAGAAGAAA | Start: 295 |
| Tm | 59.753 | 60.038 | End: 518 |
| Size | 20 | 20 | 224 |
(TCTTG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGCCCATTCAGCTTTTC | GACGGCCGGAGAAGAAGAAA | Start: 294 |
| Tm | 60.109 | 60.038 | End: 518 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TTTCTTCTTCTCCGGCCGTC | AGACAAATCGTCCTCGGCTC | Start: 499 |
| Tm | 60.038 | 59.826 | End: 609 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | CGGTGCCCATTCAGCTTTTC | CCTGTACGAATGCAGCTCCA | Start: 294 |
| Tm | 60.109 | 60.108 | End: 472 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | TGGAGCTGCATTCGTACAGG | AGACAAATCGTCCTCGGCTC | Start: 453 |
| Tm | 60.108 | 59.826 | End: 609 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | GGTGCCCATTCAGCTTTTCC | GACGGCCGGAGAAGAAGAAA | Start: 295 |
| Tm | 59.753 | 60.038 | End: 518 |
| Size | 20 | 20 | 224 |
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