ssr_primer
Primer Details for TRINITY_DN86505_c2_g1_i2 |
|---|
| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCTCTCTTCCGTCATCCT | TCAGCGCTGAAGTCATGGTT | Start: 3 |
| Tm | 60.033 | 59.965 | End: 167 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | AGCCTCTCTTCCGTCATCCT | GGGCATTCTGGGAGATGCTT | Start: 3 |
| Tm | 60.033 | 60.106 | End: 245 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CAGCCTCTCTTCCGTCATCC | TCAGCGCTGAAGTCATGGTT | Start: 2 |
| Tm | 59.896 | 59.965 | End: 167 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
|---|
| Sequence | AGCCTCTCTTCCGTCATCCT | ACGAATGATGTCAGCGCTGA | Start: 3 |
| Tm | 60.033 | 60.109 | End: 177 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
|---|
| Sequence | AAGCATCTCCCAGAATGCCC | CGAGATTTGGGCCTCACAGT | Start: 226 |
| Tm | 60.106 | 60.036 | End: 469 |
| Size | 20 | 20 | 244 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCTCTCTTCCGTCATCCT | TCAGCGCTGAAGTCATGGTT | Start: 3 |
| Tm | 60.033 | 59.965 | End: 167 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | AGCCTCTCTTCCGTCATCCT | GGGCATTCTGGGAGATGCTT | Start: 3 |
| Tm | 60.033 | 60.106 | End: 245 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CAGCCTCTCTTCCGTCATCC | TCAGCGCTGAAGTCATGGTT | Start: 2 |
| Tm | 59.896 | 59.965 | End: 167 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
|---|
| Sequence | AGCCTCTCTTCCGTCATCCT | ACGAATGATGTCAGCGCTGA | Start: 3 |
| Tm | 60.033 | 60.109 | End: 177 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
|---|
| Sequence | AAGCATCTCCCAGAATGCCC | CGAGATTTGGGCCTCACAGT | Start: 226 |
| Tm | 60.106 | 60.036 | End: 469 |
| Size | 20 | 20 | 244 |
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