ssr_primer
Primer Details for TRINITY_DN86658_c3_g1_i12 |
|---|
| (AG)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGCCTGGGGTTTATGTTC | CCAACAATCCCCGAGACCAA | Start: 242 |
| Tm | 60.035 | 59.962 | End: 521 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | GCCAGCACTTTAGCCAGAGA | ATAAAGGCCGCAAGAGGTCC | Start: 580 |
| Tm | 60.036 | 60.107 | End: 833 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | TGATGCCAGCACTTTAGCCA | ATAAAGGCCGCAAGAGGTCC | Start: 576 |
| Tm | 59.962 | 60.107 | End: 833 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | CTCGGCCTGGGGTTTATGTT | AGCCATGCACTTGGGAAGAA | Start: 241 |
| Tm | 60.034 | 59.887 | End: 494 |
| Size | 20 | 20 | 254 |
| Primer 5 details |
|---|
| Sequence | CTCGGCCTGGGGTTTATGTT | AAGAGCCATGCACTTGGGAA | Start: 241 |
| Tm | 60.034 | 59.887 | End: 497 |
| Size | 20 | 20 | 257 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGCCTGGGGTTTATGTTC | CCAACAATCCCCGAGACCAA | Start: 242 |
| Tm | 60.035 | 59.962 | End: 521 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | GCCAGCACTTTAGCCAGAGA | ATAAAGGCCGCAAGAGGTCC | Start: 580 |
| Tm | 60.036 | 60.107 | End: 833 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | TGATGCCAGCACTTTAGCCA | ATAAAGGCCGCAAGAGGTCC | Start: 576 |
| Tm | 59.962 | 60.107 | End: 833 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | CTCGGCCTGGGGTTTATGTT | AGCCATGCACTTGGGAAGAA | Start: 241 |
| Tm | 60.034 | 59.887 | End: 494 |
| Size | 20 | 20 | 254 |
| Primer 5 details |
|---|
| Sequence | CTCGGCCTGGGGTTTATGTT | AAGAGCCATGCACTTGGGAA | Start: 241 |
| Tm | 60.034 | 59.887 | End: 497 |
| Size | 20 | 20 | 257 |
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