ssr_primer
Primer Details for TRINITY_DN86849_c6_g5_i1 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTCTCGGCACAAGGATGA | AATACTCACCACTGGCAGGC | Start: 284 |
| Tm | 60.037 | 60.035 | End: 410 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | GCTGGTGTCATGGCAGTTTG | TCATCCTTGTGCCGAGACAC | Start: 71 |
| Tm | 60.039 | 60.037 | End: 303 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | ATTCAGCTCCAGGCATCACC | CCTGGAAACAAAGCTGTGGC | Start: 2356 |
| Tm | 60.107 | 59.967 | End: 2539 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | GTGTCTCGGCACAAGGATGA | ACAACGCCCCACTTTAACCA | Start: 284 |
| Tm | 60.037 | 60.107 | End: 486 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | ACGTTGAATGAGGCTGGTGT | TCATCCTTGTGCCGAGACAC | Start: 59 |
| Tm | 59.891 | 60.037 | End: 303 |
| Size | 20 | 20 | 245 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTCTCGGCACAAGGATGA | AATACTCACCACTGGCAGGC | Start: 284 |
| Tm | 60.037 | 60.035 | End: 410 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | GCTGGTGTCATGGCAGTTTG | TCATCCTTGTGCCGAGACAC | Start: 71 |
| Tm | 60.039 | 60.037 | End: 303 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | ATTCAGCTCCAGGCATCACC | CCTGGAAACAAAGCTGTGGC | Start: 2356 |
| Tm | 60.107 | 59.967 | End: 2539 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | GTGTCTCGGCACAAGGATGA | ACAACGCCCCACTTTAACCA | Start: 284 |
| Tm | 60.037 | 60.107 | End: 486 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | ACGTTGAATGAGGCTGGTGT | TCATCCTTGTGCCGAGACAC | Start: 59 |
| Tm | 59.891 | 60.037 | End: 303 |
| Size | 20 | 20 | 245 |
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