ssr_primer
Primer Details for TRINITY_DN87253_c0_g1_i6 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACGGACTGGCTCCAATTG | ATGGTATGAAACAGCGGCCA | Start: 341 |
| Tm | 60.036 | 60.035 | End: 529 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | CAGACGGACTGGCTCCAATT | ATGGTATGAAACAGCGGCCA | Start: 340 |
| Tm | 60.036 | 60.035 | End: 529 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GCAATGATGAGCTCGCCAAG | CATACGTTGGTGGAGGGGAC | Start: 800 |
| Tm | 59.971 | 60.108 | End: 1043 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | GCAATGATGAGCTCGCCAAG | GACATACGTTGGTGGAGGGG | Start: 800 |
| Tm | 59.971 | 60.108 | End: 1045 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | TCGCTAGGAGAATTGCCGAC | ATGGTATGAAACAGCGGCCA | Start: 371 |
| Tm | 59.898 | 60.035 | End: 529 |
| Size | 20 | 20 | 159 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACGGACTGGCTCCAATTG | ATGGTATGAAACAGCGGCCA | Start: 341 |
| Tm | 60.036 | 60.035 | End: 529 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | CAGACGGACTGGCTCCAATT | ATGGTATGAAACAGCGGCCA | Start: 340 |
| Tm | 60.036 | 60.035 | End: 529 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GCAATGATGAGCTCGCCAAG | CATACGTTGGTGGAGGGGAC | Start: 800 |
| Tm | 59.971 | 60.108 | End: 1043 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | GCAATGATGAGCTCGCCAAG | GACATACGTTGGTGGAGGGG | Start: 800 |
| Tm | 59.971 | 60.108 | End: 1045 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | TCGCTAGGAGAATTGCCGAC | ATGGTATGAAACAGCGGCCA | Start: 371 |
| Tm | 59.898 | 60.035 | End: 529 |
| Size | 20 | 20 | 159 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACGGACTGGCTCCAATTG | ATGGTATGAAACAGCGGCCA | Start: 341 |
| Tm | 60.036 | 60.035 | End: 529 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | CAGACGGACTGGCTCCAATT | ATGGTATGAAACAGCGGCCA | Start: 340 |
| Tm | 60.036 | 60.035 | End: 529 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GCAATGATGAGCTCGCCAAG | CATACGTTGGTGGAGGGGAC | Start: 800 |
| Tm | 59.971 | 60.108 | End: 1043 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | GCAATGATGAGCTCGCCAAG | GACATACGTTGGTGGAGGGG | Start: 800 |
| Tm | 59.971 | 60.108 | End: 1045 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | TCGCTAGGAGAATTGCCGAC | ATGGTATGAAACAGCGGCCA | Start: 371 |
| Tm | 59.898 | 60.035 | End: 529 |
| Size | 20 | 20 | 159 |
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