ssr_primer
Primer Details for TRINITY_DN87351_c2_g3_i1 |
|---|
| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTCTTCTGGTCATGGCTT | GGAGTGAGTTGCCATCGGAA | Start: 1006 |
| Tm | 60.035 | 60.036 | End: 1232 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
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| Sequence | CCAACCATTTGCAGTGGAGC | AAGCCATGACCAGAAGAGGC | Start: 829 |
| Tm | 60.038 | 60.035 | End: 1025 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TTCCGATGGCAACTCACTCC | GAAAATGCCAGCACCCACAG | Start: 1213 |
| Tm | 60.036 | 60.038 | End: 1408 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | GCGAGGAGGATGACAGTCAG | GCTCCACTGCAAATGGTTGG | Start: 655 |
| Tm | 59.898 | 60.038 | End: 848 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
|---|
| Sequence | CTGCGAGGAGGATGACAGTC | GCTCCACTGCAAATGGTTGG | Start: 653 |
| Tm | 59.898 | 60.038 | End: 848 |
| Size | 20 | 20 | 196 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTCTTCTGGTCATGGCTT | GGAGTGAGTTGCCATCGGAA | Start: 1006 |
| Tm | 60.035 | 60.036 | End: 1232 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | CCAACCATTTGCAGTGGAGC | AAGCCATGACCAGAAGAGGC | Start: 829 |
| Tm | 60.038 | 60.035 | End: 1025 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TTCCGATGGCAACTCACTCC | GAAAATGCCAGCACCCACAG | Start: 1213 |
| Tm | 60.036 | 60.038 | End: 1408 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | GCGAGGAGGATGACAGTCAG | GCTCCACTGCAAATGGTTGG | Start: 655 |
| Tm | 59.898 | 60.038 | End: 848 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
|---|
| Sequence | CTGCGAGGAGGATGACAGTC | GCTCCACTGCAAATGGTTGG | Start: 653 |
| Tm | 59.898 | 60.038 | End: 848 |
| Size | 20 | 20 | 196 |
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