ssr_primer
Primer Details for TRINITY_DN87443_c0_g3_i2 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCCAAGGCTTCATGCGGAA | CTGCCGAAACCCACCAAATG | Start: 1164 |
| Tm | 60.034 | 60.038 | End: 1307 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | ACCAAAGGCTGTAGACGTCG | TTCCGCATGAAGCCTTGGAT | Start: 915 |
| Tm | 60.039 | 60.034 | End: 1183 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
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| Sequence | CATTTGGTGGGTTTCGGCAG | CTCAGCAGGAACTTCCGGTT | Start: 1288 |
| Tm | 60.038 | 59.965 | End: 1526 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
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| Sequence | ATCCAAGGCTTCATGCGGAA | CCGCAGTTACACACACAACG | Start: 1164 |
| Tm | 60.034 | 60.043 | End: 1344 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
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| Sequence | CGTTGTGTGTGTAACTGCGG | CTCAGCAGGAACTTCCGGTT | Start: 1325 |
| Tm | 60.043 | 59.965 | End: 1526 |
| Size | 20 | 20 | 202 |
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