ssr_primer
Primer Details for TRINITY_DN87696_c0_g1_i5 |
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| (A)33: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTTTCCAGAGCTGCGACA | AGCCACGTGCATGAGAAGAA | Start: 535 |
| Tm | 59.965 | 59.965 | End: 671 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
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| Sequence | ATGTTTCCAGAGCTGCGACA | CTACGCTCTCCCTGTTTGCA | Start: 535 |
| Tm | 59.965 | 60.037 | End: 639 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
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| Sequence | ATGTTTCCAGAGCTGCGACA | ACCTTCTACGCTCTCCCTGT | Start: 535 |
| Tm | 59.965 | 59.96 | End: 644 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | ATGTTTCCAGAGCTGCGACA | GCCACGTGCATGAGAAGAAC | Start: 535 |
| Tm | 59.965 | 59.833 | End: 670 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
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| Sequence | TGTTTCCAGAGCTGCGACAA | AGCCACGTGCATGAGAAGAA | Start: 536 |
| Tm | 60.179 | 59.965 | End: 671 |
| Size | 20 | 20 | 136 |
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