ssr_primer
Primer Details for TRINITY_DN87700_c0_g1_i6 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTCCACACTGACCACTTC | CCACGGACACTACCAATGCT | Start: 114 |
| Tm | 59.965 | 60.036 | End: 266 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | AGCATTGGTAGTGTCCGTGG | GGAAAGTGCTGCTCTCTGGT | Start: 247 |
| Tm | 60.036 | 59.964 | End: 441 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | AGCATTGGTAGTGTCCGTGG | GCTGCTCTCTGGTGTAGCAA | Start: 247 |
| Tm | 60.036 | 60.037 | End: 434 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | ATTGCACGGAGCTCGAAAGA | TTCGAGCCTCGCATTGAGTT | Start: 907 |
| Tm | 60.037 | 60.037 | End: 1024 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | CCCTCCACACTGACCACTTC | ATGGGATTTTGGCCGTTCCT | Start: 114 |
| Tm | 59.965 | 59.959 | End: 316 |
| Size | 20 | 20 | 203 |
(A)41: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTCCACACTGACCACTTC | CCACGGACACTACCAATGCT | Start: 114 |
| Tm | 59.965 | 60.036 | End: 266 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | AGCATTGGTAGTGTCCGTGG | GGAAAGTGCTGCTCTCTGGT | Start: 247 |
| Tm | 60.036 | 59.964 | End: 441 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | AGCATTGGTAGTGTCCGTGG | GCTGCTCTCTGGTGTAGCAA | Start: 247 |
| Tm | 60.036 | 60.037 | End: 434 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | ATTGCACGGAGCTCGAAAGA | TTCGAGCCTCGCATTGAGTT | Start: 907 |
| Tm | 60.037 | 60.037 | End: 1024 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | CCCTCCACACTGACCACTTC | ATGGGATTTTGGCCGTTCCT | Start: 114 |
| Tm | 59.965 | 59.959 | End: 316 |
| Size | 20 | 20 | 203 |
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