ssr_primer
Primer Details for TRINITY_DN87816_c1_g1_i4 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAGCAAGGGTTCAGAGGA | AATCCAGGAAACAGCACGGT | Start: 234 |
| Tm | 58.86 | 59.89 | End: 412 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
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| Sequence | AACAAGCAAGGGTTCAGAGGA | AATCCAGGAAACAGCACGGT | Start: 233 |
| Tm | 59.505 | 59.89 | End: 412 |
| Size | 21 | 20 | 180 |
| Primer 3 details |
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| Sequence | GGAGTTCTGCTTTCTGCTGC | TCCTCTGAACCCTTGCTTGT | Start: 7 |
| Tm | 59.479 | 58.86 | End: 253 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
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| Sequence | GCTGGAGTTCTGCTTTCTGC | TCCTCTGAACCCTTGCTTGT | Start: 4 |
| Tm | 59.479 | 58.86 | End: 253 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
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| Sequence | ACAAGCAAGGGTTCAGAGGA | CAATCCAGGAAACAGCACGG | Start: 234 |
| Tm | 58.86 | 59.477 | End: 413 |
| Size | 20 | 20 | 180 |
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