ssr_primer
Primer Details for TRINITY_DN87819_c2_g1_i3 |
|---|
| (AGA)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCAGTGTTCCCCGGTATA | AGAGGGGGCAAAAATGGGAG | Start: 16 |
| Tm | 59.957 | 59.958 | End: 291 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
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| Sequence | ACCCAGTGTTCCCCGGTATA | AGGGGGCAAAAATGGGAGTT | Start: 16 |
| Tm | 59.957 | 59.807 | End: 289 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
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| Sequence | ACCCAGTGTTCCCCGGTATA | GAGGGGGCAAAAATGGGAGT | Start: 16 |
| Tm | 59.957 | 60.252 | End: 290 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | GCGTGAAAATGGTGACGGTT | AGAGGGGGCAAAAATGGGAG | Start: 61 |
| Tm | 59.69 | 59.958 | End: 291 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
|---|
| Sequence | GCGTGAAAATGGTGACGGTT | CAGACACAAAGAGGGGGCAA | Start: 61 |
| Tm | 59.69 | 60.179 | End: 300 |
| Size | 20 | 20 | 240 |
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