ssr_primer
Primer Details for TRINITY_DN87831_c4_g1_i5 |
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| (A)25(ACAAACAAACAAACAAACAAACAAAATTGGCCTTCTTTATAACATGGTGAAAACAAAAATACATATATATACATAAAAAAAAAACTGAAAACCCTGCCCTCCATTCCTGTCCTCACAAGGGTTCCTCCATTTCATGTGTGTGGTAAGCAACCACAAGGCAAAGTTTTTGCACCCCTGGCTCAACCTCTTCCCATTTCAAATGCCAGGCGTAGGGTAGGTGGGGGTAAAAGTACGCATTTTTTCGTTTTTTCCGTATTTCCGTGACCGAATCGACTTTTAAGGCGGGAAAAGTGGCCAGGCACCTACTACAAGCCCTCGCGCACAAATTCCCAGCGAGGTCCAAATTTAGAAATAAAATCCATGGCCGTGACAAATTTTTTAAAAAAATTTGAAAATGCGTACTTTTGCCCCATTTCATGGGGTAAAAGTACGCAGGTGAAATTTCA)(AACA)5aaattggccttctttataacatggtgaaaacaaaaatacatatatatacat(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGTCCTCACAAGGGTTCC | TTACCCCCACCTACCCTACG | Start: 545 |
| Tm | 59.963 | 60.032 | End: 666 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
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| Sequence | TCATTCTGGGAGCGGAAACC | TCATGCTCACGGTTCTGCTT | Start: 105 |
| Tm | 60.035 | 59.965 | End: 295 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
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| Sequence | CTGAAAACCCTGCCCTCCAT | TTACCCCCACCTACCCTACG | Start: 524 |
| Tm | 59.96 | 60.032 | End: 666 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
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| Sequence | TGATGGTGGGCACGATTTCA | TCATGCTCACGGTTCTGCTT | Start: 188 |
| Tm | 59.963 | 59.965 | End: 295 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | TCATTCTGGGAGCGGAAACC | TGAAATCGTGCCCACCATCA | Start: 105 |
| Tm | 60.035 | 59.963 | End: 207 |
| Size | 20 | 20 | 103 |
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